Bull Vet Inst Pulawy 55, 767-770, 2011

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ANALYTICAL PROCEDURE FOR THE QUANTITATIVE DETERMINATION OF GLYCEROLTRIHEPTANOATE

EWELINA KOWALCZYK, ALEKSANDRA GRELIK, ZBIGNIEW OSIŃSKI, AND KRZYSZTOF KWIATEK

Analytical procedure for determination of glyceroltriheptanoate (GTH) in processed animal by-products was developed. Samples of meat and bone meal were sieved through 1 mm sieve, extracted with petroleum ether, and cleaned up on amino bonded solid phase extraction cartridges. Extracts were analysed with a gas chromatograph combined with flame ionisation detector, on DB5 capillary column (30 m, 0.25 mm, 0.25 µm). The whole procedure was validated. Validation parameters, i.e., recovery in the range 80%-91%, depending on the substance concentration, repeatability 2.17%-8.79%, and linearity R 2 >0.999, confirmed the suitability of the method for determination of GTH in processed animal by-products.

Key words: animal by-products, glyceroltriheptanoate, gas chromatography.