Bull Vet Inst Pulawy 55, 755-759, 2011

full text

DETERMINATION OF 17β-OESTRADIOL AND TESTOSTERONE IN BOVINE SERUM WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY

BARBARA WOŹNIAK, IWONA MATRASZEK-ŻUCHOWSKA, AND JAN ŻMUDZKI

A simple and sensitive method was developed for determination of 17β-oestradiol and testosterone in bovine serum. The hormones were analysed and quantified as heptafluorobutyric anhydride derivatives by gas chromatography–mass spectrometry after extraction from serum without any cleanup step. The method was validated according to the requirements listed in the Commission Decision 2002/657/EC. Decision limit (CCα) and detection capability (CCβ) were based on the detection of the most abundant ion and the values: 0.011µg L ^-1 and 0.018 µg L ^-1 for 17β-oestradiol were obtained, respectively. The repeatability for the validation level 0.040 µg L ^-1 was 19.2% and within laboratory reproducibility – 25.8%. For testosterone, CCα and CCβ were 0.056 µg L ^-1 and 0.096 µg L ^-1 alternately with repeatability and reproducibility below 12%. The values of the detection limits of 17β-oestradiol and testosterone were found to be below the recommended concentration, set at 0.1 µg L ^-1 and 0.5 µg L ^-1 , respectively. The method was successfully applied in the official control of hormone residues in Poland.

Key words: serum, 17β-oestradiol, testosterone, GC-MS