Bull Vet Inst Pulawy 55, 717-720, 2011

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DETERMINATION OF SULFAGUANIDINE IN MEDICATED FEEDINGSTUFFS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

MONIKA PRZENIOSŁO-SIWCZYŃSKA, EWELINA KOWALCZYK, AND KRZYSZTOF KWIATEK

A liquid chromatography-mass spectrometric method (LC-MS) for the determination of sulfaguanidine in medicated feedingstuffs was developed and validated. The samples were extracted using acidified methanol, centrifuged, and a portion of the extract was diluted with water. An appropriately diluted aliquot of the extract was analysed on a column 150 mm x 4.6 mm, 5 µm using a mixture of 0.1% formic acid in water and in acetonitrile as the mobile phase. The LC-MS was used in the positive atmospheric pressure chemical ionisation MS mode. The LC-MS method was validated for its linearity, precision, and specificity. Recovery from spiked samples was from 90.4% to 96.0% depending on sulfaguanidine concentration. The method was used for the quantitative determination of sulfaguanidine and its homogeneity in commercial medicated feedingstuffs.

Key words: sulfaguanidine, medicated feedingstuffs, liquid chromatography-mass spectrometry.