Bull Vet Inst Pulawy 55, 649-655, 2011

full text

ALNUS FIRMA SUPPLEMENTATION DIET ON HAEMATOLOGY AND INNATE IMMUNE RESPONSE IN OLIVE FLOUNDER AGAINST TENACIBACULUM MARITIMUM

RAMASAMY HARIKRISHNAN, MAN-CHUL KIM, JU-SANG KIM, DONG-HWI KIM, SEUNG-HYUN HONG, AND MOON-SOO HEO

The effect of 0%, 0.01%, 0.1%, and 1.0% supplementation of diets with Alnus firma on haematological and innate immune parameters in olive flounder (Paralichthys olivaceus) against Tenacibaculum maritimum infection was investigated. The supplemented diets were given for 30 d and then the fish were infected with T. maritimum. The particular diets were continued for 1, 2, and 4 weeks after the infection. The white blood cell count did not significantly change after any diet in the first week, whereas in 2 nd and 4 th week it was significantly increased in fish fed 0.1% and 1.0% supplementation diets. The number of red blood cells did not significantly change in fish fed 0.01% and 0.1% supplementation diets in any weeks but it was significantly increased in 1.0% supplementation diet. The haemoglobin and haematocrit levels did not significantly change after any diet in the first week; however, significantly increased in fish fed 0.1%, and 1.0% supplementation diets for 2 and 4 weeks. The biochemical parameters such as total protein and cholesterol levels significantly increased in fish fed 0.1% and 1.0% supplementation diets in the 2 nd and 4 th week, whereas in case of calcium levels the changes were observed from weeks 1 to 4. The glucose level significantly increased in all diets in weeks 2 and 4. The respiratory burst activity and lysozyme activity significantly were enhanced in 0.1% and 1.0% supplementation diets from weeks 1 to 4 compared to 0% diet. The cumulative mortality was lower in groups fed 0.1% and 1.0% supplemented diets than in the 0.01% diet. This study reported that 0.1% and 1.0% Alnus firma-supplemented diet protected the olive flounder against T. maritimum infection due to positive haematological, biochemical, and innate immune parameter changes.

Key words: Paralichthys olivaceus, Alnus firma, feed additive, Tenacibaculum maritimum, immunity