Bull Vet Inst Pulawy 55, 635-639, 2011

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DETECTION OF ENTEROHAEMORRHAGIC ESCHERICHIA COLI BY TRIPLEX REAL TIME PCR METHOD

AGATA BIELAWSKA–DRÓZD, PIOTR CIEŚLIK, MICHAŁ BARTOSZCZE, TOMASZ MIRSKI, JACEK OSEK , AND ROMUALD GRYKO

Triplex real time PCR method for detection of enterohaemorrhagic strains of Escherichia coli in meat samples was described. Total of 36 strains belonging to different serogroups were used. All strains were cultured in brain heart infusion broth and sorbitol MacConkey agar plates under aerobic conditions. Sets of primers and TaqMan probes were prepared using GenBank resources. Using the designed method we were able to detect bacteria in meat samples and determine genotypes for stx1, stx2, and eaeA genes in tested strains, simultaneously. The described method provides a rapid detection of single colonies of this pathogen in minced meat samples.

Key words: meat, enterohaemorrhagic Escherichia coli, genotypes, triplex real time PCR.